• There are many proteins circulating in the plasma part of your blood.
• The total protein test measures albumin which is the main protein, accounting for about 60 per cent of proteins in the blood.
• It also measures globulin proteins which include antibodies and enzymes.
• The test is done when investigating any one of a wide range of health conditions, including liver disease, kidney disease, nutritional status and inflammatory conditions.
• Protein tests alone do not diagnose health conditions, but they can provide important information to help pinpoint a health problem.

Blood plasma is the liquid part of the blood. A pale-yellow fluid, it represents about half of the content of whole blood, and it contains water, salts and proteins. Many different proteins float in the plasma. Proteins are important building blocks of all cells and tissues.

The total protein test measures two types of proteins:

• Albumin, which is made in the liver and accounts for about 60 per cent of protein in the blood.
• Globulins, which are proteins other than albumin.

Albumin helps carry many small molecules through the blood such as hormones and drugs, but its main purpose is to keep fluid within the blood vessels and stop it from leaking out.

Globulin proteins include enzymes, transport proteins and antibodies. They are important for immune function including inflammation, and blood clotting. Except for antibodies and some other immune system proteins, most globulins are also made in the liver.

The total protein test measures all proteins in the blood. The globulins fraction is estimated by a calculation, not by measuring globulins individually. The calculation subtracts the measured albumin value from the total protein to provide an estimate of the globulins.

Globulins are a mixed group of many proteins. Measuring them individually would require specialised tests. The calculated globulin gives a useful estimate. If the calculated globulin is abnormal, further tests may be used including protein electrophoresis and immunoglobulins (IgG, IgA, IgM) for more detailed information.

The total protein test is performed along with other tests. It gives a general snapshot of health and is commonly used to help assess:

• Liver function such as chronic liver disease, cirrhosis and hepatitis.
• Kidney health such as chronic kidney diseases and loss of protein through the kidneys.
• Nutritional status such as protein deficiency in frail and elderly adults and hospital patients, and malabsorption, as in coeliac disease.
• Infection or inflammation, including Inflammatory Bowel Disease (IBD).
• Immune system disorders including autoimmune diseases where changes in the globulin fraction may occur in conditions such as rheumatoid arthritis, Systemic Lupus Erythematosus (SLE) and Sjogren’s disease.
• Some cancers such as multiple myeloma, some lymphomas and leukaemia. Also, metastatic liver cancer.

Sample

Blood.

Any preparation?

None.

The information from protein tests can provide clues that a disease or condition is affecting protein production or a loss of protein, but the results usually do not provide a definitive diagnosis. Instead, additional follow-up tests are needed to investigate the cause. If total protein test results are not in what is considered to be the healthy range, further tests may be needed.

Reading your test report
Your results will be presented along with those of your other tests on the same form. You will see separate columns or lines for each of these tests.

Protein tests alone do not diagnose health conditions, but they can provide important information to help pinpoint the problem. Once a diagnosis has been confirmed, they are often used to monitor your condition and to make sure that any treatment you are having is working.

Reference intervals - comparing your results to the healthy population
Your results will be compared to reference intervals (sometimes called a normal range).

• Reference intervals are the range of results expected in healthy people.
• They are used to provide a benchmark for interpreting a patient's test results.
• When compared against them, your results may be flagged high or low if they sit outside this range.
• Some reference intervals are harmonised or standardised, which means all labs in Australia use them.
• Others are not because for these tests, labs are using different instruments and chemical processes to analyse samples.
• Always compare your lab results to the reference interval provided on the same report.

If your results are flagged as high or low this does not necessarily mean that anything is wrong. It depends on your personal situation.

Prolonged application of a tourniquet or standing during blood collection can increase total protein levels.

Many medications may affect total protein levels, including oestrogens, steroids and oral contraceptives. Tell your healthcare practitioner all the prescription or over-the-counter medications, supplements or illicit substances you may be taking.

The choice of tests your doctor makes will be based on your medical history and symptoms. It is important that you tell them everything you think might help.

You play a central role in making sure your test results are accurate. Do everything you can to make sure the information you provide is correct and follow instructions closely.

Talk to your doctor about any medications you are taking. Find out if you need to fast or stop any particular foods or supplements. These may affect your results. Ask:

• Why does this test need to be done?
• Do I need to prepare (such as fast or avoid medications) for the sample collection?
• Will an abnormal result mean I need further tests?
• How could it change the course of my care?
• What will happen next, after the test?

Pathology and diagnostic imaging reports can be added to your My Health Record. You and your healthcare provider can now access your results whenever and wherever needed.

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